lens culinaris agglutinin  (Vector Laboratories)

Journal: Cell metabolism

Article Title: Cytoarchitectural multi-depot profiling reveals immune-metabolic crosstalk in human colon-associated adipose tissue

doi: 10.1016/j.cmet.2025.12.008

Figure Lengend Snippet: (A) Uniform manifold approximation and projection displaying adipocyte identities and expression densities for main marker genes in the STx data. (B) Dot plots displaying the scaled average expression (expr.) profiles of the top three marker genes for each adipocyte identity in the present work and a previous study. (C) Abundance (abun.) and depot enrichments (scaled proportions, prop.) for the three main adipocyte identities. Significant enrichments ( p < 0.05 using likelihood ratio test, LRT) are indicated. (D) Heatmaps displaying expressional enrichments of Adipo SAA markers across depots. Results are shown as scaled average expression for bulk transcriptomics and proteomics from Krieg et al., as well as STx and snRNA-seq in the main (1) and validation (2) cohorts. (E) Quantification of CES1 and lectin (LCA) normalized mean fluorescence intensity based on tissue sections from six individuals imaged using confocal microscopy. Asterisks denote statistically significant differences (* p < 0.05) comparing depots using one-way repeated measures ANOVA and Tukey’s multiple comparisons test. Error bars are standard error of the mean. (F) Representative image of epiploic WAT stained with LCA and an antibody directed against CES1. Scale bars, 200 μm (merge) and 100 μm (inlay). epi, epiploic; mc, mesocolic; ms, mesenteric; om, omental; sc, subcutaneous.

Article Snippet: Fluorescein-labeled lectin (1:200, FL-1041, Vector labs) or Dylight649-labeled lectin (1:200, DL-1048–1, Vector labs), together with Hoechst33342 (1:2000, ab228551, Abcam), was applied on the slides for 20 minutes.

Techniques: Expressing, Marker, Transcriptomics, Biomarker Discovery, Fluorescence, Confocal Microscopy, Staining